Mass Production of Vesicular Arbuscular Mycorhizzal (VAM) Fungus
(VAM) are the most common nonpathogenic beneficial fungus associated with crop plants
They help in energy storage and facilitate ready exchange of nutrients
Helps in Better absorption of P and Zn
Helps in protection from Parasitic fungi and nematode
Very useful in horticultural crop nursery production.
Micropropagation Technology for the Rapid Multiplication of Triploid Seedless Watermelon
Seedlessness in watermelon is a highly value trait for the consumers industry
The tissue culture protocol would allow clonal propagation of the seedless variety
The yield of micropropogated plants is comparable to diploid parental line with similar fruit
size, quality with added advantage of seedlessness
Micropropagation of Anthurium
Technology helps in reducing the cost of production
High rate of proliferation through this method is novelty and also seedlings could be made available in 9 months
Micropropagation of Gerbera
A tissue-culture based technology for the rapid clonal propagation of elite and new gerbera lines.
A commercial multiplication of Gerbera using shoot tip has been standardized.
Potential for multiplication of millions of plants per year without the occurrence of vitrified shoots.
Micropropagation of Orchids
A tissue-culture based technology for the rapid clonal propagation of Dendrobium “Queen Sonia".
A commercial multiplication of Dendrobium “Queen Sonia using protocorm Like Bodies (PLB’s) has been standardized.
Potential for multiplication millions of plants per year without the occurrence of chimeric plants.
Micropropagation of Hippeastrum
Protocol for large scale propagation of selected pre-release hybrid of Hippeastrum using bulb scale explants (both single and twin scale) has been standardized.
Proliferation of shoots could be achieved by subculturing the bulblets generated in the same medium. There was no need of a rooting medium as the same proliferating medium could induce root formation.
Large scale propagation in a short time (high multiplication rate)
Micropropagation of Tuberose
Micropropagation of tuberose achieved through axillary shoot propagation.
A viable protocol for large scale propagation using scale stem sections which gives a multiplication rate of more than 5 shoots / explant
Protocol for double type of tuberose (Suvasini) developed for first time
Microbial Strains as Biofertilisers and Biocontrol Agents
Efficient nitrogen fixing strains of Azotobacter, Azospirillum
Phosphate solubilising strains:
Beneficial for maintaining the sustainability of soil.
Help in nitrogen fixing, solubilising phosphorus, producing hormones and growth promotion
They are useful in controlling the pathogens
Micropropagation of Banana (cvs Elakki Bale)
Invitro Micropropagation of cvs Elakki bale and Nanjungud Rasbale were achieved using suckers as explants
MS basal medium supplemented with different growth regulators were used
The regenerated shoots started multiplying from the 2nd subculture and were multiplied upto 5 – 6 cycles
Multiplication rate was 4- 5 from an individual shoots in both the cultivars
Development of Chitinase Gene Construct
Trichoderma harzianum chitinase gene construct has been developed using the chitinase gene isolated from local isolates of T. harzianum for use in plant transformation for fungal resistance.
chitinase gene isolated through RT-PCR is expressed through constitutive promoter CaMV 35S in the pBIN binary vector
The efficacy gene was demonstrated in vitro through pathogen inhibition studies and in vivo through enhanced resistance to blight in tobacco.
Micropropagation of Male Sterile Lines of Cauliflower
A protocol for Micropropagation of GMS plants has been developed for two male sterile lines of cauliflower using immature curd and shoot tip explants.
This technique is useful in identifying Male Sterile lines without markers.
Micropropagation of Pointed Gourd
Protocol for rapid in vitro multiplication of two female cultivars (`Swarna Alaukik' and `Swarna Rekha') with multiplication rate of 6.6 and 4.9x and one male line of pointed gourd (5.3x)has been developed.
The significant aspect is that a single medium formulation facilitated culture initiation, multiplication and rooting in all the genotypes. Further, the rooted stumps left after the subculture of nodes could be effectively used for ex vitro establishment.
in vitro propagation gives a much higher multiplication rate at a shorter duration (25 acclimatised plants per explant in 4 months).
Regeneration of Banana cv. Nanjungud Rasbale (Silk group Rasthali AAB) through Embryogenic Cell Suspension using male flowers
Using immature male flowers, embryogenic cell suspension (ECS) has been developed for Nanjungud Rasbale (Silk group Rasthali AAB).
The ideal calli was identified and on transfer to suitable media to give rise to cell suspensions.
From one ml of ECS around 1500 plantlets can be formed.
Plants developed through ECS are found to be superior in fruit size.